The physiological polyphosphate as a healing biomaterial for chronic wounds: Crucial roles of its antibacterial and unique metabolic energy supplying properties

Müller, Werner E. G.; Schepler, Hadrian; Neufurth, Meik

doi:https://doi.org/10.1016/j.jmst.2022.07.018

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BioAcyl Corp

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Müller, W. E. G., Schepler, H., & Neufurth, M. (2022). The physiological polyphosphate as a healing biomaterial for chronic wounds: Crucial roles of its antibacterial and unique metabolic energy supplying properties. Journal of Materials Science & Technology.
Added by: Dr. Enrique Feoli (15/08/2022, 18:19) Last edited by: Dr. Enrique Feoli (07/01/2024, 12:41)

Resource type: Journal Article
DOI: https://doi.org/10.1016/j.jmst.2022.07.018
ID no. (ISBN etc.): 1005-0302
BibTeX citation key: 2022b
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Categories: BioAcyl Corp
Subcategories: Microenvironment
Creators: Müller, Neufurth, Schepler
Collection: Journal of Materials Science & Technology

Views: 3/369

Abstract

Insufficient metabolic energy, in the form of adenosine triphosphate (ATP), and bacterial infections are among the main causes for the development of chronic wounds. Previously we showed that the physiological inorganic polymer polyphosphate (polyP) massively accelerates wound healing both in animals (diabetic mice) and, when incorporated into mats, in patients with chronic wounds. Here, we focused on a hydrogel-based gel formulation, supplemented with both soluble sodium polyP (Na-polyP) and amorphous calcium polyP nanoparticles (Ca-polyP-NP). Exposure of human epidermal keratinocytes to the gel caused a significant increase in extracellular ATP level, an effect that was even enhanced when Na-polyP was combined with Ca-polyP-NP. Furthermore, it is shown that the added polyP in the gel is converted into a coacervate, leading to encapsulation and killing of bacteria. The data on human chronic wounds showed that the administration of hydrogel leads to the complete closure of these wounds. Histological analysis of biopsies showed an increased granulation of the wounds and an enhanced microvessel formation. The results indicate that the polyP hydrogel, due to its properties to entrap bacteria and generate metabolic energy, is a very promising formulation for a new therapy for chronic wounds.

Notes

Schematic outline of the transformations/phase transitions of polyP in the hydroxyethyl cellulose-based hydrogel. (A) Formation of “Ca-polyP-NP” from Na-polyP in the hydrogel. The killing of the bacteria, the coacervate formation, and the ATP generation at the border between the injured tissue and the wound gel are sketched. (B) Coacervate formation from Na-polyP in the gel and Ca2+ extruded from the wound exudate causing the death of bacteria. (C) Formation of ATP via AMP and ADP from polyP driven by the enzymes ALP and ADK.

Added by: Dr. Enrique Feoli Last edited by: Dr. Enrique Feoli