Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals

Grifoni, A.; Weiskopf, D.; Ramirez, S. I.; Mateus, J.; Dan, J. M.; Moderbacher, C. R.; Rawlings, S. A.; Sutherland, A.; Premkumar, L.; Jadi, R. S.; Marrama, D.; de Silva, A. M.; Frazier, A.; Carlin, A. F.; Greenbaum, J. A.; Peters, B.; Krammer, F.; Smith, D. M.; Crotty, S.; Sette, A.

doi:10.1016/j.cell.2020.05.015

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Grifoni, A., Weiskopf, D., Ramirez, S. I., Mateus, J., Dan, J. M., & Moderbacher, C. R., et al. (2020). Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals. Cell, 181(7), 1489–1501.e15.
Added by: Dr. Enrique Feoli (17/10/2020, 19:01) Last edited by: Dr. Enrique Feoli (17/10/2020, 19:02)

Resource type: Journal Article
DOI: 10.1016/j.cell.2020.05.015
ID no. (ISBN etc.): 0092-8674
BibTeX citation key: Grifoni2020
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Categories: BioAcyl Corp
Subcategories: Cross immunity
Creators: Carlin, Crotty, Dan, de Silva, Frazier, Greenbaum, Grifoni, Jadi, Krammer, Marrama, Mateus, Moderbacher, Peters, Premkumar, Ramirez, Rawlings, Sette, Smith, Sutherland, Weiskopf
Collection: Cell

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Abstract

{Understanding adaptive immunity to SARS-CoV-2 is important for vaccine development, interpreting coronavirus disease 2019 (COVID-19) pathogenesis, and calibration of pandemic control measures. Using HLA class I and II predicted peptide “megapools,” circulating SARS-CoV-2-specific CD8+ and CD4+ T cells were identified in ∼70% and 100% of COVID-19 convalescent patients, respectively. CD4+ T cell responses to spike, the main target of most vaccine efforts, were robust and correlated with the magnitude of the anti-SARS-CoV-2 IgG and IgA titers. The M, spike, and N proteins each accounted for 11%–27% of the total CD4+ response, with additional responses commonly targeting nsp3, nsp4, ORF3a, and ORF8, among others. For CD8+ T cells, spike and M were recognized, with at least eight SARS-CoV-2 ORFs targeted. Importantly, we detected SARS-CoV-2-reactive CD4+ T cells in ∼40%–60% of unexposed individuals, suggesting cross-reactive T cell recognition between circulating “common cold” coronaviruses and SARS-CoV-2. Graphical Abstract Download : Download high-res image (143KB)Download : Download full-size image}