BioAcyl Corp
BioAcyl Corp |
|
 |
 |
 |
|
Pascual-Reguant, A., Kroh, S., & Hauser, A. E. (2024). Tissue niches and immunopathology through the lens of spatial tissue profiling techniques. European Journal of Immunology, 54(2), 2350484. Added by: Dr. Enrique Feoli (21/07/2025, 18:23) Last edited by: Dr. Enrique Feoli (29/07/2025, 19:48) |
 |
| Resource type: Journal Article DOI: 10.1002/eji.202350484 BibTeX citation key: PascualReguant2024 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Perivascular microenvironment Keywords: COVID-19, immunopathology, multiplex histology, spatial transcriptomics, tissue niches Creators: Hauser, Kroh, Pascual-Reguant Collection: European Journal of Immunology |
Views: 4/25
|
| Abstract |
|
Abstract Spatial organization plays a fundamental role in biology, influencing the function of biological structures at various levels. The immune system, in particular, relies on the orchestrated interactions of immune cells with their microenvironment to mount protective or pathogenic immune responses. The COVID-19 pandemic has underscored the significance of studying immunity within target organs to understand disease progression and severity. To achieve this, multiplex histology and spatial transcriptomics have proven indispensable in providing a spatial context to protein and gene expression patterns. By combining these techniques, researchers gain a more comprehensive understanding of the complex interactions at the cellular and molecular level in distinct tissue niches, key functional units modulating health and disease. In this review, we discuss recent advances in spatial tissue profiling techniques, highlighting their advantages over traditional histopathology studies. The insights gained from these approaches have the potential to revolutionize the diagnosis and treatment of various diseases including cancer, autoimmune disorders, and infectious diseases. However, we also acknowledge their challenges and limitations. Despite these, spatial tissue profiling offers promising opportunities to improve our understanding of how tissue niches direct regional immunity, and their relevance in tissue immunopathology, as a basis for novel therapeutic strategies and personalized medicine.
Added by: Dr. Enrique Feoli Last edited by: Dr. Enrique Feoli |
| Notes |
Spatial fingerprints of biological units. The spatial organization of biological structures or units determines their function: (1) the subcellular localization of molecules, such as genes, transcripts and proteins, within a cell, (2) the precise location of cells within tissues, (3) the microanatomical arrangement of distinct tissue niches, composed of several interdependent cells, (4) the positioning of tissues or organs within an individual, and (5) the spatial distribution of individuals and populations around the world. The spatial fingerprints at each of these scales are interconnected. Only through spatial analysis of the different levels of human biology (from molecular biology to epidemiology), will we reach a comprehensive understanding of health and disease.
Activated fibrovascular niches host immune-stromal interactions that direct lung immunopathology in severe COVID-19 disease. Endothelial disruption upon SARS-CoV-2 infection leads to the leakage of heme into the lung tissue. CD163+ heme-scavenger macrophages accumulate in the lungs during chronic COVID-19 disease and secrete CCL18. This chemokine promotes endothelial-to-mesenchymal (EndMT) transition via its cognate receptor, CCR8, which is expressed in the activated vessel wall. In prolonged COVID-19 disease, the activated fibrovascular niche up-regulates CCL21, which promotes (1) EndMT via CCR7, also expressed in the activated vascular wall, (2) the recruitment and/or retention of CCR7+ exhausted/stem-like T cells, and (3) ultimately the formation of tertiary lymphoid organs. The schematic summarizes the findings from Ref. [8] and exemplifies how the combination of spatial profiling techniques can reveal mechanisms of tissue immunopathology. Added by: Dr. Enrique Feoli Last edited by: Dr. Enrique Feoli |
