BioAcyl Corp
BioAcyl Corp |
|
 |
 |
 |
|
Kangawa, K., Minamino, N., & Chino, N. (1981). The complete amino acid sequence of α-neo-endorphin. Biochemical and Biophysical Research Communications, 99(3), 871–878. Added by: Dr. Enrique Feoli (14/10/2023, 17:14) Last edited by: Dr. Enrique Feoli (14/10/2023, 17:16) |
 |
| Resource type: Journal Article DOI: https://doi.org/10.1016/0006-291X(81)91244-4 ID no. (ISBN etc.): 0006-291X BibTeX citation key: Kangawa1981 View all bibliographic details  |
Categories: BioAcyl Corp Creators: Chino, Kangawa, Minamino Collection: Biochemical and Biophysical Research Communications |
Views: 2/36
|
| Abstract |
|
After re-purification by reverse phase high performance liquid chromatography, α-neo-endorphin was submitted to structural analyses, performed by dansyl-Edman degradation, as well as by C-terminal analysis by 3H-labeling. The full sequence of α-neo-endorphin has been determined to be : Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys, in which the 8th residue previously reported as Arg is found to be Tyr. A synthetic decapeptide with the above sequence was verified to be identical with natural α-neo-endorphin. For further structural confirmation, tryptic and chymotryptic peptides were also identified. Thus, the complete sequence of α-neo-endorphin has been definitely established. Its potent opioid activity in the guinea-pig ileum assay is also discussed.
|