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Yamazaki, E., Yazawa, S., & Shimada, T. (2023). Activation of SARS-CoV-2 by trypsin-like proteases in the clinical specimens of patients with COVID-19. Scientific Reports, 13(1), 11632. Added by: Dr. Enrique Feoli (22/07/2023, 15:06) Last edited by: Dr. Enrique Feoli (24/07/2023, 15:56) |
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| Resource type: Journal Article DOI: 10.1038/s41598-023-38757-8 ID no. (ISBN etc.): 2045-2322 BibTeX citation key: Yamazaki2023 View all bibliographic details  |
Categories: BioAcyl Corp, BioAcyl Corp Subcategories: COVID-19, Oral Health and COVID-19 Creators: Shimada, Yamazaki, Yazawa Collection: Scientific Reports |
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| Abstract |
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SARS-CoV-2 enters host cells through the angiotensin converting enzyme 2 (ACE2) receptor and/or transmembrane protease, serine 2 (TMPRSS2). In this study, we investigated whether proteases increased SARS-CoV-2 infectivity using pseudotyped viruses and clinical specimens from patients with COVID-19. First, we investigated how trypsin increased infectivity using the pseudotyped virus. Our findings revealed that trypsin increased infectivity after the virus was adsorbed on the cells, but no increase in infectivity was observed when the virus was treated with trypsin. We examined the effect of trypsin on SARS-CoV-2 infection in clinical specimens and found that the infectivity of the SARS-CoV-2 delta variant increased 36,000-fold after trypsin treatment. By contrast, the infectivity of SARS-CoV-2 omicron variant increased to less than 20-fold in the clinical specimens. Finally, using five clinical specimens containing delta variants, enhancement of viral infectivity was evaluated in the presence of the culture supernatant of several anaerobic bacteria. As a result, viral infectivities of all the clinical specimens containing culture supernatants of Fusobacterium necrophorum were significantly increased from several- to tenfold. Because SARS-CoV-2 infectivity increases in the oral cavity, which may contain anaerobic bacteria, keeping the oral cavities clean may help prevent SARS-CoV-2 infection.
Added by: Dr. Enrique Feoli Last edited by: Dr. Enrique Feoli |
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Number: 1 Publisher: Nature Publishing Group
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