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Caire, R., Audoux, E., & Thomas, M. (2022). YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitro. Nature Communications, 13(1), 6995. Added by: Dr. Enrique Feoli (26/04/2023, 18:56) Last edited by: Dr. Enrique Feoli (26/04/2023, 18:59) |
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| Resource type: Journal Article DOI: 10.1038/s41467-022-34432-0 ID no. (ISBN etc.): 2041-1723 BibTeX citation key: Caire2022 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Constitutive innate immunity Creators: Audoux, Caire, Thomas Collection: Nature Communications |
Views: 1/191
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| Abstract |
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Transcriptional cofactors YAP/TAZ have recently been found to support autophagy and inflammation, which are part of cell-autonomous immunity and are critical in antibacterial defense. Here, we studied the role of YAP against Staphylococcus aureus using CRISPR/Cas9-mutated HEK293 cells and a primary cell-based organoid model. We found that S. aureus infection increases YAP transcriptional activity, which is required to reduce intracellular S. aureus replication. A 770-gene targeted transcriptomic analysis revealed that YAP upregulates genes involved in autophagy/lysosome and inflammation pathways in both infected and uninfected conditions. The YAP-TEAD transcriptional activity promotes autophagic flux and lysosomal acidification, which are then important for defense against intracellular S. aureus. Furthermore, the staphylococcal toxin C3 exoenzyme EDIN-B was found effective in preventing YAP-mediated cell-autonomous immune response. This study provides key insights on the anti-S. aureus activity of YAP, which could be conserved for defense against other intracellular bacteria.
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Number: 1 Publisher: Nature Publishing Group
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