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Le Bert, N., Tan, A. T., Kunasegaran, K., Tham, C. Y. L., Hafezi, M., & Chia, A., et al. (2020). SARS-CoV-2-specific T cell immunity in cases of COVID-19 and SARS, and uninfected controls. Nature, 1–10. Added by: Dr. Enrique Feoli (16/07/2020, 12:43) Last edited by: Dr. Enrique Feoli (16/07/2020, 12:59) |
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| Resource type: Journal Article Peer reviewed DOI: 10.1038/s41586-020-2550-z ID no. (ISBN etc.): 1476-4687 BibTeX citation key: LeBert2020 View all bibliographic details  |
Categories: Zotero Subcategories: Inmunidad de mucosas Creators: Bertoletti, Chen, Chia, Chia, Chng, Hafezi, Kalimuddin, Kunasegaran, Le Bert, Lin, Linster, Low, Ooi, Tambyah, Tan, Tan, Tan, Tham, Wang Collection: Nature |
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| Abstract |
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Memory T cells induced by previous pathogens can shape the susceptibility to, and clinical severity of, subsequent infections1. Little is known about the presence of pre-existing memory T cells in humans with the potential to recognize SARS-CoV-2. Here, we first studied T cell responses to structural (nucleocapsid protein, NP) and non-structural (NSP-7 and NSP13 of ORF1) regions of SARS-CoV-2 in COVID-19 convalescents (n=36). In all of them we demonstrated the presence of CD4 and CD8 T cells recognizing multiple regions of the NP protein. We then showed that SARS-recovered patients (n=23) still possess long-lasting memory T cells reactive to SARS-NP 17 years after the 2003 outbreak, which displayed robust cross-reactivity to SARS-CoV-2 NP. Surprisingly, we also frequently detected SARS-CoV-2 specific T cells in individuals with no history of SARS, COVID-19 or contact with SARS/COVID-19 patients (n=37). SARS-CoV-2 T cells in uninfected donors exhibited a different pattern of immunodominance, frequently targeting the ORF-1-coded proteins NSP7 and 13 as well as the NP structural protein. Epitope characterization of NSP7-specific T cells showed recognition of protein fragments with low homology to “common cold” human coronaviruses but conserved amongst animal betacoranaviruses. Thus, infection with betacoronaviruses induces multispecific and long-lasting T cell immunity to the structural protein NP. Understanding how pre-existing NP- and ORF-1-specific T cells present in the general population impact susceptibility and pathogenesis of SARS-CoV-2 infection is of paramount importance for the management of the current COVID-19 pandemic.
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Publisher: Nature Publishing Group
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