BioAcyl Corp
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Kusumoto, T., Yotsukura, M., & Asakura, T. (2025). Induced lung epithelial-like cells derived by direct reprogramming rescue influenza virus-induced lung injury in mice. Biochemical and Biophysical Research Communications, 778, 152384. Added by: Dr. Enrique Feoli (24/12/2025, 18:22) Last edited by: Dr. Enrique Feoli (24/12/2025, 18:27) |
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| Resource type: Journal Article DOI: 10.1016/j.bbrc.2025.152384 ID no. (ISBN etc.): 0006-291X BibTeX citation key: Kusumoto2025 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Cell reprogramming Keywords: Alveolar epithelial cells, Cell therapy, Direct reprogramming, Influenza, Surfactant protein (SP)–C Creators: Asakura, Kusumoto, Yotsukura Collection: Biochemical and Biophysical Research Communications |
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| Abstract |
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We recently reported that a combination of four transcription factors (Nkx2-1, Foxa1, Foxa2, and Gata6) directly reprograms mouse embryonic fibroblasts (MEFs) into differentiated self-renewable alveolar epithelial-like cells in a serum-free 3D organoid system. Here, we aimed to generate induced pulmonary epithelial-like cells in serum-containing culture (iPULsSC) using the same four transcription factors in a serum-containing 3D culture system. We found that the global gene expression profile of iPULsSC was similar to that of alveolar epithelial type II (AT2) and alveolar type I (AT1) cells. Surfactant protein (SP)–C-positive iPULsSC displayed lamellar body-like structures, consistent with the features of AT2 cells. Furthermore, we provide evidence that intratracheal administration of iPULsSC rescued influenza virus-induced acute lung injury in mice. These findings suggest that iPULsSC can provide a potential source of lung epithelial cells for regenerative medicine. Reprogramming fibroblasts using serum-containing media, besides previously established serum-free systems, can provide a potential source of lung epithelial cells.
Added by: Dr. Enrique Feoli Last edited by: Dr. Enrique Feoli |