BioAcyl Corp
BioAcyl Corp |
|
 |
 |
 |
|
Aragona, M., Sifrim, A., & Malfait, M. (2020). Mechanisms of stretch-mediated skin expansion at single-cell resolution. Nature, 584(7820), 268–273. Added by: Dr. Enrique Feoli (11/04/2025, 21:50) Last edited by: Dr. Enrique Feoli (11/04/2025, 21:54) |
 |
| Resource type: Journal Article DOI: 10.1038/s41586-020-2555-7 ID no. (ISBN etc.): 0028-0836 BibTeX citation key: Aragona2020 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Dermotaxis Creators: Aragona, Malfait, Sifrim Collection: Nature |
Views: 1/15
|
| Abstract |
|
The ability of the skin to grow in response to stretching has been exploited in reconstructive surgery. Although the response of epidermal cells to stretching has been studied in vitro ,, it remains unclear how mechanical forces affect their behaviour in vivo. Here, we develop a mouse model in which the consequences of stretching on skin epidermis can be studied at single-cell resolution. Using a multidisciplinary approach that combines clonal analysis with quantitative modelling and single-cell RNA-seq, we show that stretching induces skin expansion by creating a transient bias in the renewal activity of epidermal stem cells (SC), while a second subpopulation of basal progenitors remains committed to differentiation. Transcriptional and chromatin profiling identifies how cell states and gene regulatory networks are modulated by stretching. Using pharmacological inhibitors and mouse mutants, we define the step-by-step mechanisms that control stretch-mediated tissue expansion at single-cell resolution in vivo.
|