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Komura, S., Satake, T., & Goto, A. (2020). Induced pluripotent stem cell-derived tenocyte-like cells promote the regeneration of injured tendons in mice. Scientific Reports, 10(1), 3992. Added by: Dr. Enrique Feoli (11/11/2023, 16:41) Last edited by: Dr. Enrique Feoli (11/11/2023, 16:43) |
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| Resource type: Journal Article DOI: 10.1038/s41598-020-61063-6 ID no. (ISBN etc.): 2045-2322 BibTeX citation key: Komura2020 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Sports medicine Creators: Goto, Komura, Satake Collection: Scientific Reports |
Views: 1/88
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| Abstract |
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Tendons are dense fibrous structures that attach muscles to bones. Healing of tendon injuries is a clinical challenge owing to poor regenerative potential and scarring. Here, we created reporter mice that express EGFP, driven by the promoter of the tendon-specific Scleraxis (Scx) transcription-factor gene; we then generated induced pluripotent stem cells (iPSCs) from these mice. Utilising these fluorescently labelled iPSCs, we developed a tenogenic differentiation protocol. The iPSC-derived EGFP-positive cells exhibited elevated expression of tendon-specific genes, including Scx, Mohawk, Tenomodulin, and Fibromodulin, indicating that they have tenocyte-like properties. Finally, we demonstrated that these cells promoted tendon regeneration in mice after transplantation into injured tendons reducing scar formation via paracrine effect. Our data demonstrate that the tenogenic differentiation protocol successfully provided functional cells from iPSCs. We propose that pluripotent stem cell-based therapy using this protocol will provide an effective therapeutic approach for tendon injuries.
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Number: 1 Publisher: Nature Publishing Group
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